Identification Of Degradation Products Of Cellulase Enzyme Activity In Various Conditions Of Fungicides

Abstract

The inhibitory effects of two fungicides, m- dinitrobenzene and pentachloro phenol were studied on purified carboxymethyl cellulase enzymes, isolated from the fungus Aspergillus niger. The biodegradation products were identified by thin - layer chromatography (TLC), using the solvent system as Acetone : Water : Chloroform : Methanol (8 : 0.5 : 1 : 1, vvvv). Sugars were identified by spraying p - anisidine hydrochloric acid sol. or 20% sulfuric acid in methanol on three ascents developed. It was found that the degradation products of enzymatic hydrolysis of substrate are glucose n cellobiose, as indicated by the Rf values.